Antigen

Antigen Class

TFs and others

Antigen

Hdac1

Cell type

Cell type Class

Neural

Cell type

Basal Ganglia

MeSH Description

Large subcortical nuclear masses derived from the telencephalon and located in the basal regions of the cerebral hemispheres.

Sample

source_name

Basal Ganglia

strain

CD1

age

E13.5

chip antibody

Bethyl, A300-713A

Sequenced DNA Library

library_name

GSM5827351

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP: Cells were treated with 5mM DSG followed by 1% PFA prior to generating nuclear extracts. Extracts were sonicated for 10 minutes prior to adding listed antibody to pull down bound DNA Libraries were constructed using Nugen Ovation Ultralow DR Multiplex System and size selected in the range of 180-400bp

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

36513614

Reads aligned (%)

98.1

Duplicates removed (%)

22.0

Number of peaks

8634 (qval < 1E-05)

mm9

Number of total reads

36513614

Reads aligned (%)

98.0

Duplicates removed (%)

22.1

Number of peaks

8623 (qval < 1E-05)